師資
吳柘，2005年本科畢業(yè)于青島大學(xué)。2011年博士畢業(yè)于北京大學(xué)生物技術(shù)專業(yè)，師從瞿禮嘉教授及顧紅雅教授。2008-2009年作為訪問博士生公派至日本京都大學(xué)學(xué)習(xí)。2011-2016年在英國約翰英納斯中心，任博士后及高級博士后研究員，師從Caroline Dean爵士。2016年11月-2022年11月在南方科技大學(xué)生物系任助理教授，2022年12月通過國際評審晉升長聘副教授。2023年獲得中國植物生理與分子生物學(xué)學(xué)會-衛(wèi)志明青年創(chuàng)新獎(jiǎng)，入選教育部人才計(jì)劃。作為通訊作者在Nature Genetics, Genome Biology, Plant Cell, Molecular Plant, New Phytologist, Plant Methods等主流期刊上發(fā)表論文多篇?，F(xiàn)任Science China-Life Sciences青年編委，Essays in Biochemistry 編委等。
吳柘實(shí)驗(yàn)室主要研究植物生殖的分子調(diào)控機(jī)理，重點(diǎn)探究RNA-蛋白在轉(zhuǎn)錄同時(shí)的包裝過程以及表觀遺傳調(diào)控在其中所發(fā)揮的作用及機(jī)制。在方法上，我們采用遺傳學(xué)及前沿的表觀組學(xué)技術(shù)為研究的主要手段，結(jié)合重要的生物學(xué)過程，我們希望即見樹木（單個(gè)重要的基因）又見森林（在基因組范圍內(nèi)的普遍模式和規(guī)律），揭示植物發(fā)育及基因表達(dá)調(diào)控背后的普遍規(guī)律。
研究領(lǐng)域
1. RNA結(jié)合蛋白在發(fā)育及環(huán)境響應(yīng)中的作用范式
2. 轉(zhuǎn)錄及其偶聯(lián)的RNA命運(yùn)決定機(jī)制
3. 種子形成前后表觀遺傳/RNA調(diào)控對發(fā)育程序及發(fā)育穩(wěn)健性的作用機(jī)制
研究成果
2025
27. Wu, Z.# PRC2-mediated repression of PIF4 targets beyond the on/off switch of gene expression.(2025). Developmental Cell. 60:2025-2026
26. Jang, G., Payne-Dwyer, A., Maple, R. Wu, Z., Liu, F., Lopez, S., Wang, Y., Fang, X., Leake, M., Dean, C. Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3’ processing. (2025) EMBO Journal 44: 2056 - 2074
2024
25. Menon, G., Mateo-Bonmati, E., Reeck, S., Maple, R., Zhe, W., Ietswaart, R., Dean, C., Howard, M. Proximal termination generates a transcriptional state that determines the rate of establishment of Polycomb silencing. (2024). Mol cell. 84:2255-2271.
24. Zhu, D#., Wen, Y., Tan Y., Chen, X.,# Wu, Z.# A simple, robust, cost-effective, and low-input ChIP-seq method for profiling histone modifications and Pol II in plants.(2024). New phytologist. 244:1658-1669.
2023
23. Zhu, D., Wen, Y., Yao, W., Zheng, H., Zhou, S., Zhang, Q., Qu, L.J., Chen, X.,#and Wu, Z.# (2023). Distinct chromatin signatures in the Arabidopsis male gametophyte. Nature Genetics. 55:706-720. (co-corresponding author)
22. Zhou, S., Zhao, F., Zhu, D., Zhang Q., Dai Z., Wu Z.# (2023). Coupling of co-transcriptional splicing and 3′ end Pol II pausing pausing during termination in Arabidopsis. Genome Biology. 204:206. (co-corresponding author)
21. Chen L., Xu Z., Huang J., Shu, H., Hui, Y., Zhu, D., Wu, Y., Dong, S., Wu, Z.# Plant immunity suppressor SKPR encodes a novel RNA-binding protein that targets exon 3′ end of unspliced RNA. New Phytologist. 240:1467-1483.(co-corresponding author)
2022
20. Zhang Q, Zhao F, Wu Z, Zhu D. A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis. Plant Methods. 2022, 18:135.
19. Yang D, Zhao F, Zhu D, Chen X, Kong X, Wu Y, Chen M, Du J, Qu L,-J, Wu, Z#. Progressive chromatin silencing of ABA biosynthesis genes permits seed germination in Arabidopsis. Plant Cell 2022, 34:2871-2891.
18. Tong J, Ren Z, Sun L, Zhou S, Yuan W, Hui Y, Ci D, Wang W, Fan LM, Wu Z, Qian W. ALBA proteins confer thermotolerance through stabilizing HSF messenger RNAs in cytoplasmic granules. Nat Plants 2022, 8:778-791.
17. Shang L, Li X, He H, Yuan Q, Song Y, Wei Z, Lin H, Hu M, Zhao F, Zhang C, et al: A super pan-genomic landscape of rice. Cell Res 2022.
2021
16. Xu, C*., Wu, Z*., Duan, H.C*., Fang, X., Jia, G., and Dean, C. (2021). R-loop resolution promotes co-transcriptional chromatin silencing. Nat Commun 12, 1790.
15. Wang, Q., Xue, Y., Zhang, L., Zhong, Z., Feng, S., Wang, C., Xiao, L., Yang, Z., Harris, C.J., Wu, Z., et al. (2021). Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation. Science 374, 1152-1157.
14. Zhao, N., Su, X.M., Liu, Z.W., Zhou, J.X., Su, Y.N., Cai, X.W., Chen, L., Wu, Z., and He, X.J. (2021). The RNA recognition motif-containing protein UBA2c prevents early flowering by promoting transcription of the flowering repressor FLM in Arabidopsis. New Phytol.
13. Xu, Z., Shi, X., Bao, M., Song, X., Zhang, Y., Wang, H., Xie, H., Mao, F., Wang, S., Jin, H., et al. (2021). Transcriptome-Wide Analysis of RNA m(6)A Methylation and Gene Expression Changes Among Two Arabidopsis Ecotypes and Their Reciprocal Hybrids. Front Plant Sci 12, 685189.
2020
12. Wu Z#. (2020): An introduction to the themed issue on RNA biology in China. Essays in Biochemistry, 64, 863-866.
11. Zhu D*., Mao F*., Tian Y., Lin X., Gu L., Gu H., Qu L.J., Wu Y#., and Wu Z#. (2020): The features and regulation of co-transcriptional splicing in Arabidopsis. Molecular Plant, 13, 278-294.
10. Wu Z., Fang X., Zhu D., Dean C. (2020). Autonomous pathway: FLC repression through an antisense-mediated chromatin silencing mechanism. Plant Physiology 182, 27-37.
9. Fang XF*., Wu Z*., Raitskin O, Webb K., Voigt P., Lu T., Howard M., and Dean C. (2020). The 3′ processing of antisense RNAs physically links to chromatin-based transcriptional control. PNAS, 117, 15316-15321.
Before 2017
8. Huang J., Gu L. Zhang Y., Yan T., Kong G., Kong L., Guo B., Qiu M., Wang Y., Jing M., Xing W., Ye W., Wu Z., Zhang Z., Zheng X., Gijzen M., Wang Y., Dong S. (2017). An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity. Nature Communications 8, 2051-2063.
7. Ietswaart, R.*, Rosa, S.*, Wu, Z.*, Dean, C., and Howard, M. (2017). Cell-size-dependent transcription of FLC and its antisense long non-coding RNA COOLAIR explain cell-to-cell expression variation. Cell Systems 4, 622-635 e629.
6. Wu, Z.*, Ietswaart, R.*, Liu, F., Yang, H., Howard, M., and Dean, C. (2016). Quantitativeregulation of FLC via coordinated transcriptional initiation and elongation. PNAS 113,218-223.
5. Wu, Z.*, Zhu, D.*, Lin, X.*, Miao, J.*, Gu, L., Deng, X., Yang, Q., Sun, K., Cao, X., Tsuge, T., Dean, C., Aoyama, T., Gu, H., and Qu, L.J. (2016). RNA-binding proteins RZ-1B andRZ-1C play critical roles in regulating pre-mRNA splicing and gene expression duringdevelopment in Arabidopsis. The Plant Cell 28, 55-73.
4. Wang, Z.W.*, Wu, Z.*, Raitskin, O., Sun, Q., and Dean, C. (2014). Antisense-mediated FLCtranscriptional repression requires the P-TEFb transcription elongation factor. PNAS 111,7468-7473.
3. Zhu, D.*, Wu, Z.*, Cao, G.*, Li, J., Wei, J., Tsuge, T., Gu, H., Aoyama, T., and Qu, L.J. (2014). TRANSLUCENT GREEN, an ERF family transcription factor, controls water balance in Arabidopsis by activating the expression of aquaporin genes. Molecular Plant 7,601-615.
2. Marquardt, S., Raitskin, O., Wu, Z., Liu, F., Sun, Q., and Dean, C. (2014). Functionalconsequences of splicing of the antisense transcript COOLAIR on FLC transcription.Molecular Cell 54, 156-165.
1. Ietswaart, R., Wu, Z., and Dean, C. (2012). Flowering time control: another window to the connection between antisense RNA and chromatin. Trends in Genetics 28, 445-453.